Generation of Egg Yolk Antibodies in Chicken ( IgY ) Against Influenza M 2 ( M 2 e ) Protein
نویسنده
چکیده
Chicken egg yolk immunoglobulins (IgY) from immunized hens have considerable advantages for the production of polyclonal antibodies. It reacts with more epitopes and avoids the interference in immunological assays. Regarding the IgY aptitudes, this study was conducted to produce chicken IgY specific for influenza M2 (M2e) protein. Fusion construct harboring C-terminal of bovine heat shock protein 70 (Hsp70) and influenza M2e coding genes was injected to laying hens. Egg yolk antibodies were screened for the presence of anti-M2e antibodies by indirect enzyme-linked immunosorbent assay (ELISA). Anti-M2e antibodies were detected in egg yolks of injected hens from 13 days, with the peak titer at 41 days. Anti-M2e polyclonal, monospecific IgY antibodies could be used for different areas of research, diagnostics, medical application
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